Composite

Part:BBa_K3440008:Design

Designed by: Julie Cordier, Aditi Kallai   Group: iGEM20_Stockholm   (2020-10-23)


RhlR with myc tag under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


Design Notes

This part was designed to show that RhlR can be expressed under constitutive promoter. A modearte strength RBS BBa_B0034 was used and a myc tag was added for easier characterisation.

Source

RhlR from Pseudomonas aeruginosa

References